Support Page for Waters Customers in Israel

דף תמיכה בלקוחות ווטרס בישראל

Training courses at Waters

Link to Waters Website

Download Waters’ Products Guide

Register first to Waters’ site for some of the documents הרשמה לאתר כאן

Get Support from Waters (TC) Israel:

 גישה לקבלת עזרה ותמיכה

I-REQUEST on Waters Website

Phone Support
Service Visit
On-Site Training

First: Interactive Tutorial: download the application here

מדריכי מיכשור ותוכנה צעד-צעד  Step by Step Guides

• Empower: Getting Started with GPC (Waters’ website) (you need the GPC Default project)
• Breeze Software: Getting Started with GPC (You need the GPC Default project)
• Millennium 4.0 PDA Getting Started Guide (You need the PDA Default project)
• Empower PDA Getting Started Guide   (You need the PDA Default project)
• Empower ZQ Getting Started Guide
• GC in Empower: Getting Started
• eL_PDA Getting Started Guide
• Acquity Fluorescence Detector Guide
• Quick guide to SQ detector

Empower Support Dcuments

Step by Step:

• Creating methods (Hebrew)
•  Custom Fields views Emp 2
• Integration Events (English)
• Quantitative Processing (English)
• System Suitability (Hebrew)
• Summary Reports (English)

Empower 2

• Run Samples  הרצת דוגמאות
• •Creating Methods-Emp2 יצירת שיטות
• •The Processing Method  שיטת עיבוד
• •System Suitability-Emp2
• •Exporting Table Results יצוא נתונים
  
• •Backup and Restore Projects גיבוי פרוייקטים
• •Integration Events (English)
• Custom Fields views Emp 2
• Summary_Reports_Emp2
• •Control Standard
• Dissolution_SampleSet
• •Reviewing, Baseline noise and limits in sample sets (Hebrew)

Links and Documents

• Empower 2 Compliance Assessment with 21CFR Part 11
• Empower 2 Compliance Assessment with Annex 11

• Search for Sertificate of Structural Integrity of Empower

• CALCULATIONS_Assay_Emp2 עיבוד נתונים וחישובים
• Quantitative Processing (English)GPC Processing (Millennium) Step by Step

  Setting GPC Processing Method properly for multimode MW distribution2

• •Intersample Calculations – Examples דוגמאות לחישובים בין דוגמאות

• Emp2_Baseline_Noise_Feb12 (Hebrew)

• SIGNAL-NOISE_Empower הוראות בעברית על סידור התוכנה לחישובים של יחס סיגנל לרעש

• Signal to noise in Empower 3

• USP Signal to Noise in Emp 2EP Signal to Noise in Emp 2

• EXPORTING_RAW_DATA
• •Processing Internal Standards
• •Quantitative Processing (English)
•  •Quantitative Processing – How to fill in values
• •Integration Events

 Layout of the rest of the article: Solvent delivery systemהמשאבה

• The column הקולונה
• Detector  הגלאי
• Injector   המזרק
• Gradient and isocratic  גרדיינט או איזוקרטי
• Data Processing
• עיבוד נתונים

Waters’ Chemistry Products מוצרי כימיה

• Column Selection –Advisor
• Columns Selectivity Chart
• Vials-Selector
• Plates -Advisor
• Sample-Preparation-Product-Advisor

Register הרשמה first to Waters’ website! (www.waters.com)
Waters’ Catalogue קטלוגים

Waters’ USP “L” Listing

Columns Care and Use Manuals

Care and Use Manual of Commonly Used Columns מדריכי שימוש ושמירה על קולונות

XBridge Sunfire Spherisorb

Symmetry Syragel(GPC)

Acquity HSS Xterra

Acquity BEH Cortecs Ultrahydrogel

IC_Pak Atlantis Glycane/amide

Columns and SPE Application Books pdf files

ספרי שיטות ואפליקציות של קולונות ומוצרי הכנת דוגמאות

(Files can be downloaded only after registration  יש להירשם קודם   at Waters)

HPLC Troubleshooting Guide Oasis Application Book Stability Indicating Method with Sunfire Symmetry Application notebook Oasis  96 Well Plate Presentation Environmental Application Solutions Xterra Applications Notebook Drug of Abuse Applications Application solutions for Oligonucleotides Atlantis Application Notebook Protein Separation Technologies Medicinal Chemistry Application solutions Acquity Applications book Glycan / Amide Column (replacement of Amine columns) Biopharmaceutical Application Solutions

Chemistry Products for Bio-Separations מוצרי כימיה להפרדות ביולוגיות

Operational support תמיכה תיפעולית במיכשור

Successful Operation of HPLC System מדריך להפעלה נכונה של

איתור חלקי חילוף

Waters’ parts locator

  MORE:

• מדריך להפעלת HPLC בעברית  Hebrew guide for HPLC operation (pdf 210K)
• How To Make a Phosphate Buffer
• Preparation of the Waters’ Alliance  2690/5 (English) in details פירוט הוראות להכנת מכשיר
• Step by Step Procedure for the Preparation of 2695 unit צעד צעד: הכנת מכשיר לעבודה.
• Preparation of the Waters’ 2790/5 Separation unit -Alliance HT הכנת מערכת לעבודה
• Attacking Carry-over Effects מאמר על התמודדות עם
• Maintenance of HPLC with concentrated rough samples המלצות על התמודדות עם דוגמאות קשות
• Switching between Normal phase and Reversed phase הדרכה על מעבר מהיפוך פאזות לפאזה נורמלית
• Hebrew Overview on HPLC (pdf 350k)
• Selecting water for HPLC איך לבחור מים  (Millipore’s)
• Solvent Preparation
• The importance of Temperature Control החשיבות של שליטה בטמפרטורה
• Final UPLC Method description: Example   (pdf) טופס דוגמא לכתיבה של שיטה סופית
• Column Archives: LC Troubleshooting LC-GC Europe –  By: John W. Dolan
• Waters applications for method development and validation פיתוח שיטות וולידציה באתר של ווטרס
• “Ghost” peaks: Background contamination

הסבר על הפרופילים של הגרדיינטים במשאבות

PDA support Materials – תמיכה בגלאי מערך-דיודות: חומר עזר

Overview (can be used for SOP):

• Peak Purity and Spectral Matching procedures – Experimental and software considerations (English)

Hebrew Article:

• Using Photo-Diode Array Detectors (PDA) for Spectral Analysis of chromatograms

Lecture:

• •Principles of Peak Purity and Spectral Matching

Waters Performance perspectives:

• Peak Purity I  ; Peak Purity II;  Peak Purity III;  Library Matching. 
• Instrumental Deviation from Beer’s Law

Materials for Self Training: חומרים ללימוד עצמי

Register to Waters’ website and download the pdf guide:

• Empower – Getting Started with PDA Software Guide

Another Tutorial for self – Training

• Some basic tools for working with Waters’ PDA

User Guides of most abundant systems’ components מדריכי שימוש ואחזקה במכשירים

2695: http://www.waters.com/webassets/cms/support/docs/71500269502rb.pdf
2996 PDA http://www.waters.com/webassets/cms/support/docs/71500023202rc.pdf
2487UVhttp://www.waters.com/webassets/cms/support/docs/wat048740r2.pdf
2998 PDA http://www.waters.com/webassets/cms/support/docs/71500121902rc.pdf
2489: UVhttp://www.waters.com/webassets/cms/support/docs/71500142102rb.pdf Acquity UPLC binaryhttp://www.waters.com/webassets/cms/support/docs/715002209ra.pdf Acquity PDA  http://www.waters.com/webassets/cms/support/docs/715002209ra.pdf Acquity TUV  http://www.waters.com/webassets/cms/support/docs/715002209ra.pdf 1500 series (Breeze-Plantex)  http://www.waters.com/webassets/cms/support/docs/71500022204ra.pdf
432 conductivity detector  http://www.waters.com/webassets/cms/support/docs/71500043202ra.pdf SQ MS detector http://www.waters.com/webassets/cms/support/docs/71500126602re.pdf

Selection of General Articles for Troubleshooting מאמרים נבחרים

• Troubleshooting Autosamplers Part I (Dolan)
• Troubleshooting Autosamplers Part II (Dolan)
• Troubleshooting UPLC
• ChromAcademy Troubleshooting course
• Waters’ LC Troubleshooting guide

21CFR PART 11 ISSUES

How to Prepare your Empower to 21 CFR Part11:

• Empower 1 Settings for CFR Part 11
• Empower 2 Compliance Assessment with 21CFR Part 11

• Empower 2 Compliance Assessment with Annex 11

• Technological control on 21CFRPart11

• Summary page on Data Integrity

How to Prepare Your Laboratory for HPLC הכן מעבדתך לעבודה נכונה ב

Overview (can be used for SOP):

• Peak Purity and Spectral Matching procedures – Experimental and software considerations (English)

Hebrew Article:

• Using Photo-Diode Array Detectors (PDA) for Spectral Analysis of chromatograms (Hebrew-

Lecture:

• Principles of Peak Purity and Spectral Matching – 

Waters Performance perspectives:

• Peak Purity I  ; Peak Purity II;  Peak Purity III;  Library Matching. 
• Instrumental Deviation from Beer’s Law

Materials for Self Training: חומרים ללימוד עצמי

Please download the default project for Millennium/Empower and unzip it:

•  PDA Default – Millennium Project (download, save, unzip and restore to your Millennium station) to use with:

Register to Waters’ website and download the pdf guide:

• Empower – Getting Started with PDA Software Guide

Another Tutorial for self – Training

• Some basic tools for working with Waters’ PDA

WaterSelecting water for HPLC
Solvents:

• JT Baker solvents
• Spectrum-Chemicals
• Merck HPLC/TLC solvents
• PierceNet Solvents

Buffers

• On-line recipes for Buffers
• HPLC Buffers-Sigma

Solvent and Sample handling

• Solvent Preparation
• Autosampler Vials
• Vial Selector  – Plate Selector
• Filter Selector
• Waters Inc. Filtration products (register first)
• SGE Syringes

Successful Operation of HPLC System תיפעול נכון של מערכת

Introduction

Before beginning to work with any HPLC instrument one must understand the basic principles of liquid chromatography at its various modes.

The liquid chromatograph is very complex and it consists of sophisticated technology and sometimes delicate and total ignorance in the manner it works might sabotage its parts which are very expensive.  In addition, there are chemical processes of distribution between the two phases, the mobile and stationary, while the analysis goes on, and there is a need for a minimum understanding of their theoretical basis to operate the system correctly.  Also, a minimum understanding of the method, in which the system performs data analysis, is needed so that no false data or results will be obtained in the analysis.

LISTS OF STEPS NEEDED BEFORE ANY RUN BY HPLC  רשימת צעדים מקדימים לפני הרצה :

• Filter the solvents with membranes with cutoff of 0.22-0.45 mM
• Use clean and transparent reservoirs through which precipitates and colloids can be distinguished.
• Make sure that the solvents will be easily mixed with the previous solvents in the same inlets (A, B, C, D).  For example methanol or water should not be placed instead of hexane directly, or any organic solvent should not be placed directly instead of a buffer reservoir.
• Degass the solvents and purge all the tubing that lead to the pump.
• Connect the column carefully according to the flow direction marked on it (do not connect directly to the detector).
• Flow the appropriate solvents through the column at a low flow-rate (0.1-0.5 ml/min) or reach the composition gradually using the appropriate gradient.
•  Select the appropriate wavelength (or other type of setting) in the detector and wait for stable baseline
• Prepare the set of methods in the workstation: Instrument method for the control on the system, Processing method for the data processing and the Report method for the report of final results.
• When the system and the methods are ready a blank run should be performed to test the system and verify that it is clean from interferences.

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