- Get Support from Waters (TC) Israel:
- I-REQUEST on Waters Website
- מדריכי מיכשור ותוכנה צעד-צעד Step by Step Guides
- Empower Support Dcuments
- Layout of the rest of the article: Solvent delivery systemהמשאבה The column הקולונה Detector הגלאי Injector המזרק Gradient and isocratic גרדיינט או איזוקרטי Data Processing עיבוד נתונים Waters’ Chemistry Products מוצרי כימיה Column Selection –Advisor Columns Selectivity Chart Vials-Selector Plates -Advisor Sample-Preparation-Product-Advisor Register הרשמה first to Waters’ website! (www.waters.com) Waters’ Catalogue קטלוגים
- Care and Use Manual of Commonly Used Columns מדריכי שימוש ושמירה על קולונות
- Columns and SPE Application Books pdf files
- ספרי שיטות ואפליקציות של קולונות ומוצרי הכנת דוגמאות
- Chemistry Products for Bio-Separations מוצרי כימיה להפרדות ביולוגיות
- איתור חלקי חילוף
- Materials for Self Training: חומרים ללימוד עצמי
- Materials for Self Training: חומרים ללימוד עצמי
- Successful Operation of HPLC System תיפעול נכון של מערכת
Support Page for Waters Customers in Israel
דף תמיכה בלקוחות ווטרס בישראל
Download Waters’ Products Guide
Register first to Waters’ site for some of the documents הרשמה לאתר כאן
Get Support from Waters (TC) Israel:
גישה לקבלת עזרה ותמיכה
I-REQUEST on Waters Website
Phone Support
Service Visit
On-Site Training
First: Interactive Tutorial: download the application here
מדריכי מיכשור ותוכנה צעד-צעד Step by Step Guides
- Empower: Getting Started with GPC (Waters’ website) (you need the GPC Default project)
- Breeze Software: Getting Started with GPC (You need the GPC Default project)
- Millennium 4.0 PDA Getting Started Guide (You need the PDA Default project)
- Empower PDA Getting Started Guide (You need the PDA Default project)
- Empower ZQ Getting Started Guide
- GC in Empower: Getting Started
- eL_PDA Getting Started Guide
- Acquity Fluorescence Detector Guide
- Quick guide to SQ detector
Empower Support Dcuments
Step by Step:
- Creating methods (Hebrew)
- Custom Fields views Emp 2
- Integration Events (English)
- Quantitative Processing (English)
- System Suitability (Hebrew)
- Summary Reports (English)
Empower 2
- Run Samples הרצת דוגמאות
- •Creating Methods-Emp2 יצירת שיטות
- •The Processing Method שיטת עיבוד
- •System Suitability-Emp2
- •Exporting Table Results יצוא נתונים
- •Backup and Restore Projects גיבוי פרוייקטים
- •Integration Events (English)
- Custom Fields views Emp 2
- Summary_Reports_Emp2
- •Control Standard
- Dissolution_SampleSet
- •Reviewing, Baseline noise and limits in sample sets (Hebrew)
Links and Documents
- CALCULATIONS_Assay_Emp2 עיבוד נתונים וחישובים
- Quantitative Processing (English)GPC Processing (Millennium) Step by Step
Setting GPC Processing Method properly for multimode MW distribution2
- •Intersample Calculations – Examples דוגמאות לחישובים בין דוגמאות
- Emp2_Baseline_Noise_Feb12 (Hebrew)
- EXPORTING_RAW_DATA
- •Processing Internal Standards
- •Quantitative Processing (English)
- •Quantitative Processing – How to fill in values
- •Integration Events
Layout of the rest of the article: Solvent delivery systemהמשאבה
- The column הקולונה
- Detector הגלאי
- Injector המזרק
- Gradient and isocratic גרדיינט או איזוקרטי
- Data Processing
- עיבוד נתונים
Waters’ Chemistry Products מוצרי כימיה
- Column Selection –
Advisor - Columns Selectivity Chart
- Vials-Selector
- Plates -Advisor
- Sample-Preparation-
Product-Advisor
Register הרשמה first to Waters’ website! (www.waters.com)
Waters’ Catalogue קטלוגים
Care and Use Manual of Commonly Used Columns מדריכי שימוש ושמירה על קולונות
Columns and SPE Application Books
pdf files
ספרי שיטות ואפליקציות של קולונות ומוצרי הכנת דוגמאות
(Files can be downloaded only after registration יש להירשם קודם at Waters)
|
Chemistry Products for Bio-Separations מוצרי כימיה להפרדות ביולוגיות
Operational support תמיכה תיפעולית במיכשור
Successful Operation of HPLC System מדריך להפעלה נכונה של
איתור חלקי חילוף
MORE:
- מדריך להפעלת HPLC בעברית Hebrew guide for HPLC operation (pdf 210K)
- How To Make a Phosphate Buffer
- Preparation of the Waters’ Alliance 2690/5 (English) in details פירוט הוראות להכנת מכשיר
- Step by Step Procedure for the Preparation of 2695 unit צעד צעד: הכנת מכשיר לעבודה.
- Preparation of the Waters’ 2790/5 Separation unit -Alliance HT הכנת מערכת לעבודה
- Attacking Carry-over Effects מאמר על התמודדות עם
- Maintenance of HPLC with concentrated rough samples המלצות על התמודדות עם דוגמאות קשות
- Switching between Normal phase and Reversed phase הדרכה על מעבר מהיפוך פאזות לפאזה נורמלית
- Hebrew Overview on HPLC (pdf 350k)
- Selecting water for HPLC איך לבחור מים (Millipore’s)
- Solvent Preparation
- The importance of Temperature Control החשיבות של שליטה בטמפרטורה
- Final UPLC Method description: Example (pdf) טופס דוגמא לכתיבה של שיטה סופית
- Column Archives: LC Troubleshooting LC-GC Europe – By: John W. Dolan
- Waters applications for method development and validation פיתוח שיטות וולידציה באתר של ווטרס
- “Ghost” peaks: Background contamination
הסבר על הפרופילים של הגרדיינטים במשאבות
PDA support Materials – תמיכה בגלאי מערך-דיודות: חומר עזר
Overview (can be used for SOP):
Hebrew Article:
Lecture:
Waters Performance perspectives:
- Peak Purity I
; Peak Purity II;
Peak Purity III;
Library Matching.
- Instrumental Deviation from Beer’s Law
Materials for Self Training: חומרים ללימוד עצמי
Register to Waters’ website and download the pdf guide:
Another Tutorial for self – Training
User Guides of most abundant systems’ components מדריכי שימוש ואחזקה במכשירים
2695: http://www.waters.com/webassets/cms/support/docs/71500269502rb.pdf
2996 PDA http://www.waters.com/webassets/cms/support/docs/71500023202rc.pdf
2487UVhttp://www.waters.com/webassets/cms/support/docs/wat048740r2.pdf
2998 PDA http://www.waters.com/webassets/cms/support/docs/71500121902rc.pdf
2489: UVhttp://www.waters.com/webassets/cms/support/docs/71500142102rb.pdf Acquity UPLC binaryhttp://www.waters.com/webassets/cms/support/docs/715002209ra.pdf Acquity PDA http://www.waters.com/webassets/cms/support/docs/715002209ra.pdf Acquity TUV http://www.waters.com/webassets/cms/support/docs/715002209ra.pdf 1500 series (Breeze-Plantex) http://www.waters.com/webassets/cms/support/docs/71500022204ra.pdf
432 conductivity detector http://www.waters.com/webassets/cms/support/docs/71500043202ra.pdf SQ MS detector http://www.waters.com/webassets/cms/support/docs/71500126602re.pdf
Selection of General Articles for Troubleshooting מאמרים נבחרים
- Troubleshooting Autosamplers Part I (Dolan)
- Troubleshooting Autosamplers Part II (Dolan)
- Troubleshooting UPLC
- ChromAcademy Troubleshooting course
- Waters’ LC Troubleshooting guide
21CFR PART 11 ISSUES
How to Prepare your Empower to 21 CFR Part11:
How to Prepare Your Laboratory for HPLC הכן מעבדתך לעבודה נכונה ב
Overview (can be used for SOP):
Hebrew Article:
Lecture:
Waters Performance perspectives:
- Peak Purity I
; Peak Purity II;
Peak Purity III;
Library Matching.
- Instrumental Deviation from Beer’s Law
Materials for Self Training: חומרים ללימוד עצמי
Please download the default project for Millennium/Empower and unzip it:
Register to Waters’ website and download the pdf guide:
Another Tutorial for self – Training
WaterSelecting water for HPLC
Solvents:
Buffers
Solvent and Sample handling
- Solvent Preparation
- Autosampler Vials
- Vial Selector – Plate Selector
- Filter Selector
- Waters Inc. Filtration products (register first)
- SGE Syringes
Successful Operation of HPLC System תיפעול נכון של מערכת
Introduction
Before beginning to work with any HPLC instrument one must understand the basic principles of liquid chromatography at its various modes.
The liquid chromatograph is very complex and it consists of sophisticated technology and sometimes delicate and total ignorance in the manner it works might sabotage its parts which are very expensive. In addition, there are chemical processes of distribution between the two phases, the mobile and stationary, while the analysis goes on, and there is a need for a minimum understanding of their theoretical basis to operate the system correctly. Also, a minimum understanding of the method, in which the system performs data analysis, is needed so that no false data or results will be obtained in the analysis.
LISTS OF STEPS NEEDED BEFORE ANY RUN BY HPLC רשימת צעדים מקדימים לפני הרצה :
- Filter the solvents with membranes with cutoff of 0.22-0.45 mM
- Use clean and transparent reservoirs through which precipitates and colloids can be distinguished.
- Make sure that the solvents will be easily mixed with the previous solvents in the same inlets (A, B, C, D). For example methanol or water should not be placed instead of hexane directly, or any organic solvent should not be placed directly instead of a buffer reservoir.
- Degass the solvents and purge all the tubing that lead to the pump.
- Connect the column carefully according to the flow direction marked on it (do not connect directly to the detector).
- Flow the appropriate solvents through the column at a low flow-rate (0.1-0.5 ml/min) or reach the composition gradually using the appropriate gradient.
- Select the appropriate wavelength (or other type of setting) in the detector and wait for stable baseline
- Prepare the set of methods in the workstation: Instrument method for the control on the system, Processing method for the data processing and the Report method for the report of final results.
- When the system and the methods are ready a blank run should be performed to test the system and verify that it is clean from interferences.