• High Performance Liquid Chromatography (HPLC) is an analytical technique for the separation and determination of organic and inorganic solutes in any samples especially biological, pharmaceutical, food, environmental, industrial, etc. In a liquid chromatographic process a liquid permeates through a porous solid stationary phase and elutes the solutes into a flow-through detector.
  • The stationary phase is usually in the
  • form of small-diameter (5-10 mm) uniform particles, packed into a cylindrical column.
  • The typical column is constructed from a rigid material (such as stainless steel or plastic) and is generally 5-30 cm long and the internal diameter is in the range of1-9 mm.
  • A high pressure pump is required to force the mobile phase through the column at typical flow rates of 0.1-2 ml/min.
  • The sample to be separated is introduced into the mobile phase by injection device, manual or automatic, prior to the column.
  • The detector usually contains low volume cell through which the mobile phase passes carrying the sample components.